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KMID : 0359320130530040217
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Korean Journal of Veterinary Research
2013 Volume.53 No. 4 p.217 ~ p.224
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Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice
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Ahn Dong-Joo
Youm Jung-Won
Kim Suk-Weon
Yoon Won-Kee
Kim Hyoung-Chin
Hur Tai-Young
Joung Young-Hee
Jeon Jae-Heung
Kim Hyun-Soon
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Abstract
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Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-S0.7) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-S0.7 gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-S0.7 protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-S0.7 antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.
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KEYWORD
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oral administration, potato-derived antigen, transmissible gastroenteritis virus, transgenic plant
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